Low-Intensity Ultrasound Inhibits Proliferation and Induces Apoptosis of Ovarian Cancer SKOV3 Cells and Its Mechanism
[Objective]To investigate the effects of low-intensity ultrasound(LIUS)on proliferation and apoptosis of ovarian cancer cells and its mechanism.[Methods]Ovarian cancer SKOV3 cells were treated with LIUS,and the cell viability and apoptosis were determined by CCK8 assay and cytome-try,respectively.The differentially expressed genes related to ultrasound treatment were obtained and screened with existing gene databases,and KEGG enrichment analysis and PPI network construction were performed.The progression free survival and overall survival associated with key gene expres-sions were extracted from existing cancer and prognostic databases for prognostic analysis,and the mechanisms of LIUS promoting apoptosis of ovarian cancer cells were analyzed.[Results]With the increase of LIUS irradiation time and intensity,the viability of SKOV3 cells decreased and the num-ber of apoptotic cells increased significantly in a time and intensity dependent manner with an opti-mal intensity of 2.0 W/cm2 and optimal time of 70 s.The effect was strongest when the intensity reaches 2.0 W/cm2 and optimal time of 70 s,which was considered to be the best irradiation parameter.Bioinformatics analysis showed that LIUS may promote the apoptosis of ovarian cancer cells through up-regulating YY1 and HSPA5 genes and down-regulating GRN gene.[Conclusion]LIUS can inhibit the proliferation and induce apoptosis of ovarian cancer SKOV3 cells in a time and intensity depen-dent manner,which may be associated with up-regulation of YY1 and HSPA5 genes,and down-regu-lation of the GRN gene.
万方数据
维普
