Application of Esophagography for Diagnosis of Esophageal Cancer in Established Mouse Model
[Objective]To evaluate the application of esophagography for the diagnosis of esophageal carcinoma in established animal model.[Methods]C57BU6 mice were fed with car-cinogen 4-nitroquinoline-1-oxide(4-NQO)to establish an orthotopic model of esophageal squamous cell carcinoma.After 28 weeks of modelling,the mice underwent esophagography;then the ani-mals were sacrificed and the esophageal tissues were examined by HE staining and immunohisto-chemistry.[Results]An orthotopic mouse model of esophageal cancer was successfully estab-lished after 28 weeks of feeding 4-NQO.Esophagography was performed in 4 model mice,show-ing thickening of the esophagus,obstruction of the contrast agent flow and filling defect in the tu-mor lesions.HE staining and immunohistochemical CK14 staining confirmed the esophagographic diagnosis in 2 mice with esophageal squamous cell carcinoma,and other 2 mice with esophageal squamous papilloma.[Conclusion]Esophagography can assist the diagnosis in the establishment of orthotopic mouse esophageal cancer model.
esophagographyorthotopic esophageal cancer modelesophageal squamous cell carcinomadiagnosis
NETL
NSTL
万方数据
