食管造影在诊断原位食管癌动物模型成功建立中的作用
Application of Esophagography for Diagnosis of Esophageal Cancer in Established Mouse Model
尹子豪 1张红芳 2唐荣军 3莫墨 3赵江 3张珂 3俞青青3
作者信息
- 1. 浙江中医药大学第四临床医学院,浙江杭州 310053
- 2. 杭州市肿瘤医院,杭州肿瘤研究所,浙江杭州 310002
- 3. 杭州市肿瘤医院,浙江杭州 310002
- 折叠
摘要
[目的]评估食管造影在辅助诊断食管癌原位动物模型成功建立中的作用.[方法]采用饲喂致癌物4-硝基喹啉-1-氧化物(4-NQO)法建立C57BU6小鼠食管鳞癌原位模型.28周后采用食管造影检查对模型小鼠的食管进行摄片并影像分析.分离获取模型小鼠的食管组织,采用HE染色及免疫组化法进行病理诊断,以评估食管造影的准确性及实用性.[结果]饲喂致癌物4-NQO法成功建立了食管癌小鼠原位模型.成功对4只模型小鼠完成食管造影检查.4只模型小鼠的食管均呈现食管变粗、造影剂流通受阻及"充盈缺损"现象,提示了食管病变位置.通过HE染色及食管鳞癌标志物CK14免疫组化分析明确其中2只模型小鼠的食管病变为食管鳞癌,另外2只食管鳞状乳头状瘤,肿瘤显示率100％.[结论]食管造影可以辅助诊断小鼠食管癌原位模型的成功建立.
Abstract
[Objective]To evaluate the application of esophagography for the diagnosis of esophageal carcinoma in established animal model.[Methods]C57BU6 mice were fed with car-cinogen 4-nitroquinoline-1-oxide(4-NQO)to establish an orthotopic model of esophageal squamous cell carcinoma.After 28 weeks of modelling,the mice underwent esophagography;then the ani-mals were sacrificed and the esophageal tissues were examined by HE staining and immunohisto-chemistry.[Results]An orthotopic mouse model of esophageal cancer was successfully estab-lished after 28 weeks of feeding 4-NQO.Esophagography was performed in 4 model mice,show-ing thickening of the esophagus,obstruction of the contrast agent flow and filling defect in the tu-mor lesions.HE staining and immunohistochemical CK14 staining confirmed the esophagographic diagnosis in 2 mice with esophageal squamous cell carcinoma,and other 2 mice with esophageal squamous papilloma.[Conclusion]Esophagography can assist the diagnosis in the establishment of orthotopic mouse esophageal cancer model.
关键词
食管造影/食管癌原位动物模型/食管鳞癌/诊断Key words
esophagography/orthotopic esophageal cancer model/esophageal squamous cell carcinoma/diagnosis引用本文复制引用
基金项目
浙江省医学会临床科研基金项目(2021ZYC-Z05)
杭州市农业与社会发展科研项目(2020ZDSJ0552)
"大医精诚"肿瘤防治研究及学术交流公益项目()
出版年
2024
NETL
NSTL
万方数据