Expression of A Kinase Interacting Protein 1 and Transient Receptor Potential Channel 1 in Patients with Tongue Squamous Cell Carcinoma and Their Clinical Significance
[Objective]To explore the expression and clinical significance of A kinase interacting protein 1(AKIP1)and transient receptor potential channel 1(TRPC1)in patients with tongue squamous cell carcinoma(TSCC).[Methods]One hundred and ninety-four TSCC patients who underwent surgical resection with or without adjuvant radiotherapy from January 2016 to December 2019 were enrolled.The expressions of TRPC1 and AKIP1 protein and mRNA were detected by immunohistochemical staining and RT-qPCR,respectively,and patients were divid-ed into high and low expression groups.The proteins interacting with TRPC1 and AKIP1 were screened on String website,and selected by MCC methods of cytoHubba module in Cytoscape,to establish an interaction diagram.KEGG rest API was applied for functional enrichment analysis of closely related proteins.[Results]The positive rate of TRPC1 and AKIP1was 59.8％,56.2％in tumor tissues and 40.2％,33.5％in adjacent tissues of TSCC(P＜0.05),respectively.The mean values of TRPC1 and AKIP1mRNA in tumor and adjacent tissues were 2.3± 1.3,2.3±1.1 and 1.4±0.9,1.1±0.4,respectively;and the expression rates in tumor tissues were significantly higher than those in adjacent tissues(57.2％ vs 50.5％and 30.9％ vs 33.5％,P＜0.05).The expression of TRPC1 protein and mRNA showed statistical differences in patients with different N stages and TNM stages(P＜0.05).The expression of AKIP1 protein showed statistical significance in patients with different N stages and TNM stages(P＜0.05),while the expression of AKIP1 mRNA showed statistical significance in patients with different N stages,TNM stages,and pathological grading(P＜0.05).Pearson correlation analysis showed that TRPC1 protein and mRNA were positively correlated with AKIP1 protein and mRNA,respectively(P＜0.05).The survival of patients with high expression of TRPC1 and AKIP1 was significantly worse than that of patients with low expression(P＜0.05).Twenty one genes closely related to TRPC1 and AKIP1were screened ot by String website and Cytoscape soft-ware,KEGG enrichment analysis showed that these genes were mainly enriched in platelet activation,calcium signaling pathway,inflammatory regulation of TRP signaling pathway,Rap1 signaling pathway,cAMP-PKG sig-naling pathway and VEGF signaling pathway.[Conclusion]High expression of TRPC1 and AKIP1 is associated with poor prognosis of TSCC,they may participate in tumor progression through the activation of Rap1,VEGF and other signaling pathways.
tongue squamous cell carcinomaA kinase interacting protein 1transient receptor potential channel 1relationship analysis in proteinsKEGG signal enrichment analysis
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