circ_0001361 靶向 miR-486-3p 对乳腺癌细胞增殖和凋亡的影响
Effect of circ_0001361 Targeting miR-486-3p on Proliferation and Apoptosis of Breast Cancer Cell
陈冉 1吴伟伟 2王维伊1
作者信息
- 1. 江苏省肿瘤医院,江苏省肿瘤防治研究所,南京医科大学附属肿瘤医院,江苏南京 210009
- 2. 苏州大学附属第二医院,江苏苏州 215004
- 折叠
摘要
[目的]探讨circ_0001361靶向miR486-3p对乳腺癌细胞增殖和凋亡的影响.[方法]通过集落形成实验、流式细胞术、CCK-8法评估circ_0001361和miR-486-3p对乳腺癌MDA-MB-231细胞克隆、凋亡以及细胞活力的影响.双荧光素酶检测circ_0001361与miR-486-3p的相互作用.[结果]干扰circ_0001361表达显著性促进细胞凋亡(7.16％±0.65％vs 25.27％± 2.32％,P＜0.001)、上调 miR-486-3p 表达(0.96±0.06 vs 3.08±0.29,P＜0.001)、降低细胞光密度值(0.82±0.07 vs0.44±0.04,P＜0.001)和克隆形成数(84.47±7.23 vs 39.69±3.76,P＜0.001).过表达circ_0001361 显著性下调 miR-486-3p 表达(1.00±0.00 vs 0.45±0.05,P＜0.001)、促进细胞凋亡(7.36％±0.66％vs 20.44％±2.17％,P＜0.001)、降低细胞细胞光密度值(0.86±0.06 vs 0.51± 0.05,P＜0.001)和克隆形成数(89.62±7.35 vs 45.81±4.48,P＜0.001).circ_0001361 与 miR-486-3p直接结合.下调miR-486-3p表达显著性减弱干扰circ_0001361对MDA-MB-231细胞克隆形成数、凋亡率和细胞光密度值的影响(P＜0.001).[结论]干扰circ_0001361通过促进miR-486-3p表达来诱导乳腺癌细胞凋亡,并抑制其增殖.
Abstract
[Objective]To investigate the effect of circ_0001361 on the proliferation and apoptosis of breast cancer cells and its relation with miR-486-3p.[Methods]The effect of circ_0001361 and miR-486-3p on the cloning,apoptosis and cell viability of breast cancer MDA-MB-231 cells were detected by colony formation assay,flow cytometry and CCK-8 assay,respectively.The interaction between circ_0001361 and miR-486-3p was detected by dual-luciferase reporter assay.[Results]The circ_0001361 knockdown significantly promoted cell apoptosis(7.16％±0.65％vs 25.27％± 2.32％,P＜0.001),up-regulated miR-486-3p expression(0.96±0.06 vs 3.08±0.29,P＜0.001),and decreased the cell proliferation(0.82±0.07 vs 0.44±0.04,P＜0.001)and clone numbers(84.47±7.23 vs 39.69±3.76,P＜0.001).Overexpression of circ_0001361 significantly down-regulated miR-486-3p expression(1.00±0.00 vs 0.45±0.05,P＜0.001).Overexpression of miR-486-3p significantly promoted cell apoptosis(7.36％±0.66％vs 20.44％±2.17％,P＜0.001),decreased cell proliferation(0.86±0.06 vs 0.51±0.05,P＜0.001)and clone numbers(89.62±7.35 vs 45.81±4.48,P＜0.001).The circ_0001361 directly bound with miR-486-3p.Down-regulating miR-486-3p significantly reduced the effect induced by circ_0001361 interference on the colony formation numbers,apoptosis rate and proliferation of MDA-MB-231 cells(P＜0.001).[Conclusion]Interference circ_0001361 induces breast cancer cell apoptosis and inhibits cell proliferation through promoting miR-486-3p expression.
关键词
乳腺癌/circ_0001361/增殖/凋亡/miR-486-3pKey words
breast cancer/circ_0001361/proliferation/apoptosis/miR-486-3p引用本文复制引用
基金项目
江苏省卫生健康委科研项目(M2022106)
出版年
2024
NETL
NSTL
万方数据