[目的]探讨 Syncytin-1 和 Cripto-1 在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者胸腔积液外泌体中的表达及其临床意义.[方法]对41例NSCLC和41例肺炎患者的胸腔积液进行外泌体的提取、鉴定、RNA提取及Syncytin-1、Cripto-1表达的检测.通过蛋白印迹法检测外泌体蛋白表达,并利用酶联免疫吸附实验检测外泌体内蛋白含量,分析Syncytin-1和Cripto-1在NSCLC患者胸腔积液外泌体中的表达量与临床病理特征的相关性,并评估其对NSCLC的诊断效能.[结果]与肺炎患者相比,NSCLC患者的胸腔积液外泌体中Syncytin-1和Cripto-1的表达量升高(P＜0.001).蛋白印迹法检测显示外泌体表达其特异性蛋白.Syncytin-1和Cripto-1在NSCLC患者组和肺炎患者组之间存在统计学差异(P＜0.001).Syncytin-1和Cripto-1表达量对NSCLC诊断灵敏度分别为82.9％和75.6％、特异度分别为75.6％和80.5％,曲线下面积(area under curve,AUC)分别为0.847和0.849.Syncytin-1和Cripto-1诊断NSCLC的AUC值高于神经元特异性烯醇化酶和胃泌素释放肽前体(AUC分别为0.762和0.716),但低于癌胚抗原(AUC=0.931)(P＜0.001).[结论]胸腔积液外泌体中Syncytin-1、Cripto-1检测对于诊断NSCLC具有较高的灵敏度和特异度,可作为辅助检测NSCLC的新型分子标志物.
Expression of Syncytin-1 and Cripto-1 in Exosomes of Pleural Effu-sion in Non-Small Cell Lung Cancer and Their Clinical Significance
[Objective]To explore the expression of Syncytin-1 and Cripto-1 in exosomes of pleural ef-fusion in patients with non-small cell lung cancer(NSCLC)and their clinical significance.[Methods]The exosomes were extracted and identified from pleural effusion of 41 NSCLC and 41 pneumonia pa-tients.The expression of Syncytin-1 and Cripto-1 in exosomes were detected with qRT-PCR.Western blotting was used to detect exosomal protein expression,and enzyme-linked immunosorbent assay(ELISA)was used to measure the contents of exoprotein.The association of Syncytin-1 and Cripto-1 ex-pression in exosomes with clinicopathological features of NSCLC patients was analyzed,and the diag-nostic efficacy of Syncytin-1 and Cripto-1 for NSCLC was evaluated.[Results]Compared with pneu-monia patients,the expression levels of Syncytin-1 and Cripto-1 in exosomes of pleural effusion in NSCLC patients were significantly increased(P＜0.001).Western blotting analysis detected specific pro-teins in exosomes.ELISA results showed that there were significant differences in the expression of Syncytin-1 and Cripto-1 in exosome between NSCLC patients and pneumonia patients(P＜0.001).The area under the ROC curve(AUC)of Syncytin-1 and Cripto-1 for diagnosis of NSCLC were 0.847 and 0.849,with a sensitivity of 82.9％and 75.6％,specificity of 75.6％and 80.5％,respectively.The AUC values of Syncytin-1 and Cripto-1 were significantly higher than those of neuron-specific enolase and progastrin-releasing peptide(0.762 and 0.716),but lower than that of carcinoembryonic antigen(AUC=0.931)(P＜0.001).[Conclusion]The expression of Syncytin-1 and Cripto-1 in pleural effusion exosomes exhibits high sensitivity and specificity for diagnosis of NSCLC,which may be used as novel molecular markers for auxiliary diagnosis of NSCLC.
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