MicroRNA-183 Promotes Malignant Features of Bladder Cancer Cells Through Targeting LRIG1
[Objective]To investigate the effect and mechanism of microRNA-183(miR-183)on biological features of bladder cancer(BCa)cells.[Methods]The exprssions of miR-183 and leucine-rich repeats and immunoglobulin-like domains 1(LRIG1)were knocked down in human bladder cancer T24 cells.T24 cells were divided into 4 groups:NC group,miR-183 inhibior group,shLRIG1 group and miR-183 inhibitor+shLRIG1 group.The miR-183 and LRIG1 mRNA expressions were detected by qRT-PCR.The protein ex-pressions of LRIGI and epidermal growth factor receptor(EGFR)were detected by Western blot.The cell proliferation,cell migration,cell invasion and apoptosis were detected by CCK-8,Scratch test,Transwell assay and flow cytometry,respectively.[Results]Compared with NC group,LRIG1 protein expression sig-nificantly increased and EGFR expression significantly decreased in the miR-183 inhibitor group(P＜0.05),while LRIG1 protein expression significantly decreased and EGFR expression significantly increased in the shLRIG1 group(P＜0.05);the shLRIG1+miR-183 inhibitor reversed the above changes.The cell prolifera-tion,migration,invasion decreased,and the apoptosis increased in the miR-183 inhibitor group(P＜0.05),while the cell proliferation,migration,and invasion increased,and the apoptosis decreased in the shLRIG1 group(P＜0.05).There was no significant difference in the biological functions between the NC group and the shLRIG1+miR-183 inhibitor group.[Conclusion]MiR-183 may affect EGFR expression and regulate the bio-logical functions of bladder cancer cells through targeting LRIG1.
bladder neoplasmsmicroRNA-183leucine-rich repeats and immunoglobulin-like domains 1proliferationmigrationinvasionapoptosis
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