MicroRNA-183通过结合LRIG1促进膀胱癌疾病进展的研究
MicroRNA-183 Promotes Malignant Features of Bladder Cancer Cells Through Targeting LRIG1
尤泓杰 1王雍博 2刘郅涵 1沈栋杰 1吕秀依 1张栋 1严泽军1
作者信息
- 1. 宁波大学附属第一医院,浙江宁波 315010
- 2. 宁波大学附属第一医院,浙江宁波 315010;温州医科大学研究生培养基础地(宁波),浙江宁波 315300
- 折叠
摘要
[目的]探讨微小 RNA-183(microRNA-183,miR-183)对膀胱癌(bladder cancer,BCa)疾病进展的影响及作用机制.[方法]体外培养人BCa细胞株T24,分别/联合抑制细胞的miR-183和亮氨酸丰富重复免疫球蛋白样域蛋白1(leucine-rich repeats and immunoglobulin-like domains 1,LRIG1)的表达,将细胞分为对照(normal control,NC)、miR-183 inhibior、shLRIG1 和 miR-183 inhibitor+shLRIG 1 四组,qRT-PCR检测miR-183和LRIG1 mRNA的表达,Western blot检测细胞中LRIG1和表皮生长因子受体(epi-dermal growth factor receptor,EGFR)的蛋白表达水平;CCK-8、划痕实验、Transwell法及流式细胞术分别检测细胞增殖、迁移、侵袭及凋亡情况.[结果]与NC组相比,miR-183敲减(miR-183 inhibior组)的T24细胞中LRIG1的表达增加而EGFR的表达降低(P＜0.05),shLRIG1组中LRIG1表达降低而EGFR表达增加(P＜0.05),shLRIG1+miR-183 inhibitor组逆转了单个因素敲减所导致的上述变化.此外,miR-183敲减后,T24细胞的增殖、迁移、侵袭水平降低而凋亡水平增加(P＜0.05),shLRIG1组细胞的增殖、迁移、侵袭水平增加而凋亡水平降低(P＜0.05),shLRIG1+miR-183 inhibitor组与NC组比较各项指标差异均无统计学意义.[结论]miR-183可能通过靶向结合LRIG1来调控膀胱癌细胞EGFR的表达,进而影响了膀胱癌细胞的生物学功能.
Abstract
[Objective]To investigate the effect and mechanism of microRNA-183(miR-183)on biological features of bladder cancer(BCa)cells.[Methods]The exprssions of miR-183 and leucine-rich repeats and immunoglobulin-like domains 1(LRIG1)were knocked down in human bladder cancer T24 cells.T24 cells were divided into 4 groups:NC group,miR-183 inhibior group,shLRIG1 group and miR-183 inhibitor+shLRIG1 group.The miR-183 and LRIG1 mRNA expressions were detected by qRT-PCR.The protein ex-pressions of LRIGI and epidermal growth factor receptor(EGFR)were detected by Western blot.The cell proliferation,cell migration,cell invasion and apoptosis were detected by CCK-8,Scratch test,Transwell assay and flow cytometry,respectively.[Results]Compared with NC group,LRIG1 protein expression sig-nificantly increased and EGFR expression significantly decreased in the miR-183 inhibitor group(P＜0.05),while LRIG1 protein expression significantly decreased and EGFR expression significantly increased in the shLRIG1 group(P＜0.05);the shLRIG1+miR-183 inhibitor reversed the above changes.The cell prolifera-tion,migration,invasion decreased,and the apoptosis increased in the miR-183 inhibitor group(P＜0.05),while the cell proliferation,migration,and invasion increased,and the apoptosis decreased in the shLRIG1 group(P＜0.05).There was no significant difference in the biological functions between the NC group and the shLRIG1+miR-183 inhibitor group.[Conclusion]MiR-183 may affect EGFR expression and regulate the bio-logical functions of bladder cancer cells through targeting LRIG1.
关键词
膀胱肿瘤/微小RNA-183/亮氨酸丰富重复免疫球蛋白样域蛋白1/增殖/迁移/侵袭/凋亡Key words
bladder neoplasms/microRNA-183/leucine-rich repeats and immunoglobulin-like domains 1/proliferation/migration/invasion/apoptosis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据