[Objective]The aim was to evaluate the influence of mating pattern on fern reproduction.[Method]Taking Hypolepis punctata as a case,a comparative experiment of separately and mixed culturing of gametophytes was designed to compare the germination rate,survival rate,sporophyte growth and growth rate of sporophyte under two mating systems.[Result]The final survival rate of the completely homozygous sporophyte produced by separately culturing reached 62.5%,although their growth performance was weaker than that of mixed-crossing individuals.It suggests that selfing ferns can indeed lead to inbreeding depression,but still have a high ability to survive,which make a single spore has a high probability of continuing the population.Due to the special life-history characteristics of fern,we can obtain completely homozygous selfing individuals as trait-stable strains through separately culturing.[Conclusion]This study proved that the separately culturing technique is applicable in horticultural breeding.
关键词
蕨类植物/孢子培养/交配系统/纯合子/近交衰退/自交系
Key words
Fern/Spore propagation/Mating system/Homozygote/Inbreeding depression/Inbred line
维普
万方数据