Disinfection Conditions Screening and Initiation Culture Study of Rhodiola rosea Tissue Culture Seedlings in Chengde Area
[Objective]To protect Rhodiola rosea germplasm resources in Chengde area and improve their proliferation efficiency.[Method]Plant tissue culture technology was used to explore the most suitable disinfection conditions for R.rosea explants by studying the effects of different kinds and times of disinfectants,different concentrations of mercury chloride,and different combinations of alcohol and mercury chloride disinfection time on the survival rate of R.rosea stem tissue culture;By studying the effects of different types and concentrations of growth regulators on the initiation culture of R.rosea,we aim to explore the most suitable combination of growth regulators for R.rosea initiation culture.[Result]When the concentrations of 6-BA and NAA were both 1.0 mg/L,the differentiation rate and average plant height of Rhodiola tissue cultured seedlings were the highest,with a differentiation rate of 80%and an average plant height of 3.6 cm.[Conclusion]The combination ratio of 75%alcohol disinfection for 10 seconds and 0.1%mercuric chloride disinfection for 8 minutes is the most suitable for the treatment of R.rosea explants;The most suitable culture medium for starting cultivation of R.rosea is MS 6 g/L agar 30 g/L sucrose 1.0 mg/L 6-BA 1.0 mg/L NAA.
万方数据
维普
