干旱逆境下施用外源褪黑素对高粱生理调节的影响
Effects of Exogenous Melatonin Application on Physiological Regulation of Sorghum under Drought Stress
邹询 1周宇飞 2李广 1王建忠 1王佳旭 1张飞1
作者信息
- 1. 辽宁省农业科学院,辽宁沈阳 110161
- 2. 沈阳农业大学农学院,辽宁沈阳 110186
- 折叠
摘要
[目的]研究外源褪黑素对高粱幼苗干旱胁迫下的生长影响和筛选适宜浓度.[方法]以高粱品种辽糯 11 为材料,采用 15%PEG模拟干旱胁迫,设置 0、60、120、180、240 和 300 μmol/L等 6 个不同浓度梯度浸种,研究干旱胁迫下不同褪黑素浓度对高粱种子芽苗表型参数、渗透调节参数等指标的影响.[结果]不同褪黑素浓度处理下,渗透调节参数中游离氨基酸含量、脯氨酸含量、可溶性糖含量在 180 μmol/L褪黑素处理下含量最高,质膜透性在 0～180 μmol/L间不会受到损伤;抗氧化酶指标中,POD、SOD、CAT等活性指标在180 μmol/L与300 μmol/L达到最高,MDA含量与褪黑素浓度呈负相关,在180～300 μmol/L处于较低水平.[结论]外源褪黑素 180 μmol/L的浓度处理对干旱逆境下高粱芽苗生长改善效果最佳.
Abstract
[Objective]To study the effects of exogenous melatonin on the growth of sorghum seedlings under drought stress and screen the appropriate concentration.[Method]The sorghum variety Liaonuo 11 was used as the material,and 15%PEG was used to simulate drought stress.Six different concentration gradients of 0,60,120,180,240 and 300 μmol/L were set up to study the effects of different melatonin concentrations on the phenotypic parameters and osmotic adjustment parameters of sorghum seed buds and seedlings under drought stress.[Result]Under different melatonin concentrations,the contents of free amino acid,proline and soluble sugar in osmotic adjustment parameters were the highest under 180 μmol/L melatonin treatment,and the plasma membrane permeability was not damaged between 0-180 μmol/L.Among the antioxidant enzyme indexes,POD,SOD,CAT and other activity indexes reached the highest in 180 μmol/L and 300 μmol/L,MDA content was negatively correlated with melatonin concentration,and was at a low level from 180 μmol/L to 300 μmol/L.[Conclusion]The concentration of exogenous melatonin 180 μmol/L had the best effect on the growth of sorghum seedlings under drought stress.
关键词
褪黑素/高粱/干旱/抗氧化指标/渗透调节Key words
Melatonin/Sorghum/Drought/Antioxidant index/Osmotic regulation引用本文复制引用
基金项目
农产品质量安全学科建设项目(2022DD113318)
辽宁省藏粮于技重大专项(2023JH1/10200001)
辽宁省农科院院绩效后补助项目(2022-HBZ-1008)
沈阳市种业创新专项(22-318-2-15)
辽宁省农科院基本科研业务费项目(基金类)(2021QN2011)
出版年
2024
NETL
NSTL
万方数据