Improvement and Application of Phenol Staining Technique for Wheat Seed Purity Determination
[Objective]The aim was to optimize and improve the detection procedure of phenol staining method,so as to improve the operability and accuracy of seed purity detection results.[Method]The phenol staining method was improved,and the color classification of standard samples was carried out by using the phenol staining method before and after the improvement.The seed purity of wheat varieties was compared and identified,and the problems that should be paid attention to in the seed production and operation of the optimized staining method were put forward.[Result]The improved phenol staining method had strong operability and high accuracy.[Conclusion]When the phenol staining method is used to detect the purity of wheat seeds,more tests should be done for a certain variety to find out the characteristic color of this variety,in order to increase the accuracy of identification.