Study on Vitro Female Culture and Plant Regeneration in Allium fistulosum L.
[Objective]To study the induction of embryoid and regenerated plants from buds of Allium fistulosum L..[Method]In vitro gynogenetic culture of A.fistulosum L.was studied by orthogonal test.[Result]The results showed that 6-BA,NAA and 2,4-D had significant effects on gynogenesis.[Conclusion]The optimum induction medium was 6-BA 2.5 mg/L,NAA 0.04 mg/L and 2,4-D 1.0 mg/L.The survival rate of regenerated plants by gynogenetic culture can reach 78.2%.
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