[Objective]The aim was to establish a rapid propagation system for Artemisia annua through tissue culture.[Method]The sterilization effects of 75%ethanol and 0.1%mercuric chloride-tween for different durations on the disinfection of tender stems of A.annua were studied.The comparative sterilization effects of different explants and the impact of adding PPM antimicrobial agents to the basic culture medium on explant sterilization were also investigated.[Result]The results indicated that rinsing with 75%ethanol for 45 seconds followed by a 5-minute and 30-second soak in 0.1%mercuric chloride-tween solution provided the best sterilization effect.The addition of 0.1%PPM antimicrobial agent to the culture medium further reduced the contamination rate and enhanced the sterilization effect,resulting in a 40.0%mortality rate of the tender stem explants,a 32.0%contamination rate,and a 32.0%germination rate.[Conclusion]Among the explants,tender stems were found to be easier to culture than tender leaves,with a lower contamination rate,higher survival rate,and reduced complications during the cultivation process.
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