The paper aims to realize the energy utilization and harmless treatment of food waste through anaerobic fermentation technology,and uses 16S rRNA gene high-throughput sequencing technology to comprehensively analyze the composition and diversity of bacteria in the anaerobic fermentation section of food waste,as well as the key factors affecting the microbial community. The results showed that Bacteroidetes and Firmicutes played a key role in the hydrolysis and acidification of kitchen waste during the 90-day fermentation process. Thermovirga and Methanosaeta showed significant positive correlations with methane production,and were important factors affecting methane production. In the whole biogas fermentation process,the methane content is stable above 62%,therefore the gas production of the fermentation system is relatively stable. The research results can provide reference for optimizing the anaerobic digestion treatment process of kitchen waste and the environmental protection project of harmless treatment.
municipal kitchen wasteanaerobic fermentationmicrobial populationhigh-throughput sequencing of 16S rRNA genediversity change