首页|金属螯合蛋白异源表达提高E.coli对镉的生物吸附能力:修复潜力和潜在机制

金属螯合蛋白异源表达提高E.coli对镉的生物吸附能力:修复潜力和潜在机制

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镉(Cd)作为一种生物非必需的有毒重金属，通过自然排放或人类活动进入环境，对人类健康构成威胁。在微生物中高效表达金属螯合蛋白(MCP)可以增强生物对Cd的吸附能力。本研究构建了mcp基因编码的MCP的异源表达体系(GEM01)，探究了其对Cd的吸附效果和潜在机制。结果表明，Cd2+调控GEM01中mcp基因丰度，mcp基因的表达增加了Cd2+的生物吸附能力(8。09 mg/g，比对照组高2。32倍)。在GEM01自溶过程中Cd2+的保留率为87。87%。荧光光谱和分子动力学模拟表明了Cd2+与MCP之间存在较强的相互作用且Cd2+影响了MCP的二级结构。FT-IR证明了MCP中的官能团(如羧基、甲基和亚甲基)参与了MCP与Cd2+的相互作用。分子对接进一步表明MCP蛋白表面的一些极性和亲水残基(如天冬酰胺、谷氨酰胺、丝氨酸和组氨酸)通过静电引力与Cd2+结合。本研究为MCP介导的Cd2+生物修复提供了新的见解，为微生物修复重金属污染提供了基因资源。
Enhancing cadmium biosorption capacity in E.coli through heterologous expression of metal-chelating proteins:Insights into bioremediation potential and mechanisms
Cadmium(Cd)is a biologically non-essential and toxic heavy metal that enters the environment through natural emissions or anthropogenic activities,posing threats to human health.The efficient expression of metal-chelating proteins(MCP)in microorganisms can enhance microbial remediation of Cd.In this study,a heterologous expression system(GEM01)of MCP encoded by the mcp gene in E.coli was constructed,and the adsorption effect and potential mechanism on Cd were explored.The results indicated that Cd2+significantly enhanced the abundance of mcp gene in GEM01,thus increasing the Cd2+biosorption capacity(8.09 mg/g,2.32 times higher than the control).The retention of Cd2+during the autolysis of GEM01 was 87.87%.Fluorescence spectroscopy and molecular dynamics simulations demonstrated that there was a strong interaction between Cd2+and MCP.FT-IR demonstrated that some functional groups(e.g.,carboxyl group and methyl group)in MCP were involved in the interaction between MCP and Cd2+.Molecular docking further demonstrated that polar and hydrophilic residues(e.g.,aspartic acid,glutamic acid,serine,and histidine)on the surface of MCP bound to Cd2+via electrostatic attraction.These findings offer new insights into Cd2+bioremediation by MCP and genetic resources for microbial remediation of heavy metal pollution.

cadmiummcp genemetal chelating proteininteractionmicrobial remediation

武晨、吴玉俊、易盛炜、李峰

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College of Environment and Resources,Xiangtan University,Xiangtan 411105,China

Hunan Provincial University Key Laboratory for Environmental and Ecological Health,Xiangtan University,Xiangtan 411105,China

Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants,Xiangtan University,Xiangtan 411105,China

镉 mcp基因 金属螯合蛋白 相互作用 微生物修复

国家自然科学基金国家自然科学基金National Natural Science Foundation of China-Hunan Joint Fund中国博士后科学基金Hunan Provincial Innovation Foundation for Postgraduate,ChinaAid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

2217616142307521U21A202932023M742934CX20220599

2024

10.1007/s11771-024-5625-4

中南大学学报(英文版)
中南大学

中南大学学报(英文版)

CSTPCDEI
影响因子：0.47
ISSN：2095-2899
年,卷(期)：2024.31(4)
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