The best medium conbinations for callus induncing and somatic embryogenesis of Platanus occidentalis was comfirmed through our study. The 2nd or 3rd position leaves from the top were chosen and cultured in dark.MS medium with sucrose 30 g/L, agar 6 g/L, pH 5.7, NAA 0. 2 mg/L, IBA 0. 3 mg/L, 6-BA 0. 3 rng/L worked best for callus inducing, while MS medium with sucrose 40 g/L, agar 6 g/L, pH 5. 7, supplied with the hormone mixture active C, for the somatic embryogenesis.
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万方数据
维普
