Studies on quick screening of EST-SSR primers based on genomic DNA mixing pool
The screening of SSR primers for forestry is a heavy research which is very time consuming and consume intensive experimental materials. By employing genomic DNA pooling technology, a method of mixing several genomic DNA templates equivalently as the single template for PCR, was used to quickly screen SSR primers, which will significantly reduce the experimental workload and cost. Genomic DNA pooling constructed by equivalently mixing 6 Eucalyptus genomic DNA templates was used to screen 150 pairs of EST-SSR primers. The analytical results show that comparing with conventional screening method, the genomic DNA pooling method can greatly shorten experimental period and significantly reduce the consumption of genomic DNA, suggesting that this method is a fast and efficient method for screening substantive SSR primers used in trees.
NETL
NSTL
万方数据
维普
