首页|毛泡桐二倍体及其同源四倍体的AFLP和MSAP分析

毛泡桐二倍体及其同源四倍体的AFLP和MSAP分析

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  • NETL
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  • 维普
分别用AFLP和MSAP分子标记技术,研究了毛泡桐二倍体及其同源四倍体幼苗DNA碱基序列及其甲基化的变化.结果表明,毛泡桐二倍体及其同源四倍体DNA碱基序列在AFLP水平上没有发生变化;在MSAP扩增电泳凝胶上,毛泡桐二倍体及其同源四倍体分别检测到2 262和2 180个扩增位点,DNA甲基化位点占总扩增位点的比例分别为35.32％和38.58％,其中DNA全甲基化比例则为12.86％和14.13％;毛泡桐同源四倍体DNA甲基化和去甲基化频率高于其二倍体,总DNA甲基化多态性低于二倍体.
Analysis of diploid and its autotetraploid Paulownia tomentosa with AFLP and MSAP
DNA base sequence and DNA methylation of diploid and autotetraploid Paulownia tomentosa seedlings were investigated with amplified fragment length polymorphism (AFLP) and the methylation-sensitive amplification polymorphism (MSAP) respectively.The results show that there was no change of their DNA base sequences between diploid Paulownia tomentosa and its autotetraploid;there were 2 262 and 2 180 sites were detected in the diploid and its autotetraploid by MSAP,and their methylation sites were 35.32％and 38.58％ (where fully methylation rate were 12.86％ and 14.13％) respectively; DNA methylation and demethylation levels of the autotetraploid Paulownia tomentosa were higher than those of its corresponding diploid,but its total DNA polymorphism was low than the diploid.

Paulownia tomentosadiploidautotetraploidDNA methylationAFLPMSAP

翟晓巧、张晓申、范国强、赵振利、曹喜兵

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河南省林业科学研究院,河南郑州450003

河南农业大学泡桐研究所,河南郑州450002

毛泡桐 二倍体 同源四倍体 DNA甲基化 AFLP MSAP

国家自然科学基金河南省杰出人才计划项目

U1204309,30271082,30571496122101110700

2014

中南林业科技大学学报
中南林业科技大学

中南林业科技大学学报

CSTPCDCSCD北大核心
影响因子：1.442
ISSN：1673-923X
年,卷(期)：2014.34(1)
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