Phenotypic and genetic characterization of a RHD3 genetic interactor mutant rren108
Root hairs are of great agronomic importance due to their essential roles on mineral and water uptaking in plants,therefore,searching for genes involved in root hair development and their corresponding regulatory mechanisms characterization have great potentials on breeding of non-wood forest cultivars and other plant species.As a well-studied model plant,Arabidopsis (Arabidopsis thaliana) bears a lot of advantages for root hair analysis.RHD3 was identified as an important root hair regulatory player in Arabidopsis and was found to affect the root hair morphology by root hair tip growth regulation,which is also associated with defects in ER morphology.The seeds of at present,the mechanism of causing induced hairs and endoplasmic reticulum defect in rhd3 mutants is not very clear.The study intends to compare and analyze the differences of root hair and cell endoplasmic reticulum among wild Arabidopsis thaliana WT,Arabidopsis rhd3-1::GFP-HDEL mutant and rrenl08,further explores the genetic interaction factors and interaction mechanism of RHD3 regulation to root hairs.The seeds ofArabidopsis rhd3-1::GFP-HDEL mutant were treated with Ethyl Methane Sulfonate (EMS) and an stable double mutation line rren 108 was obtained.The phenotypic statistical results show that the average length of root hairs of rren108 seedlings was comparable to that of rhd3-1,but the root hair density in rren108 seedlings reduced to 29.5％ compared to that of rhd3-1;The root hairs of rren108 were also abnormal both in morphology and distribution.The genetic analysis reveals that the root hair phenotype was controlled by a single recessive nuclear gene.The examination of the ER morphology indicates that the connection points of the endoplasmic reticulum in rren108 mutants were less than that of rhd3-1 mutants,and the rren108 mutants' tubular structure became thickened and showed a blocky state,revealing that the rren108 mutants may control the occurrence of root hair through endoplasmic reticulum structure.
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NSTL
万方数据
维普
