A preliminary study on tissue culture of the rare and endangered plant Mussaenda anomala
The effect of hormone on the callus induction and differentiation of tender stem segments of Mussaenda anomala was studied. And an efficiency regeneration System of the plant was established. The results showed: the optimum medium for callus induction from leaves was MS+ 1.5 mg/L 2,4-D+0.2 mg/L6-BA. The optimum medium for callus differentiation was MS+0.5 mg/LNAA+0.5 mg/L6-BA+5 g/L cocon-utmilk. The adventitious root was induced on 1/2MS+0.5 mg/L IBA.