Determination on total flavonoids in male flowers and leaves of Eucommia ulmoides by spectrophotometry
To study the optimal method for the determination of total flavonoids content of Eucommia ulmoides,and analyze the differences of total flavonoids content in Eucommia ulmoides male flowers and leaves.The content of total flavonoids in Eucommia ulmoides male flowers and leaves were determined by NaNO2-Al(NO3)3-NaOH method and A1C13 colorimetric method,respectivly.The results showed that NaNO2-Al(NO3)3-NaOH method existed interference and the content of total flavonoids is higher,while AlCl3 colorimetric method is easy,highly reproducible,accurate,the absorbency and concentration of Rutin had a good linear in range of 5 ～ 30ug·mL-1 (R2=0.999 7),the average recovery rate was 99.27％,RSD 1.21％,and the method is suitable for mass determination of total flavonoids of Eucommia ulmoides.According to the determination results,the differences of total flavonoids content is larger among different materials and the content of total flavonoids in male flowers was obviously higher than that in leaves,which could provide a certain reference for the development and utilization ofEucommia ulmoides resources.
NETL
NSTL
万方数据
维普
