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基于SSR标记的杜仲群体遗传多样性及遗传结构

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通过分析杜仲群体的遗传多样性水平和遗传结构,旨在为杜仲资源的管理、保护和利用提供依据.利用9对基因组SSR引物对42个杜仲群体的868份种质资源进行遗传多样性分析、群体遗传结构分析、分子方差分析和聚类分析,主要结果如下:(1)遗传多样性分析表明,42个杜仲群体的遗传多样性水平均较高;(2)遗传结构分析表明,42个群体宜分为2个类群;(3)分子方差分析和聚类分析表明,杜仲的遗传变异主要在群体内,占93%,群体间的遗传变异仅占7%,群体间遗传分化水平低,相似程度高.本研究认为:杜仲群体的遗传多样性较高;杜仲群体间的遗传分化水平低,相似程度高;杜仲的遗传变异以群体内的变异为主,保护杜仲的遗传多样性时,应保存尽可能多的种群和特异单株.
Genetic diversity and genetic structure of Eucommia ulmoides populations based on SSR markers
Genetic diversity and population genetic structure were analyzed in Eucommia ulmoides Oliv.to provide useful information for management,protection and utilization.The population genetic diversity,population genetic structure,cluster analysis,and molecular variation analysis of variance(AMOVA) of 868 individuals from 42 populations was performed by using nine SSR primers.The main results are as follows:(1)The genetic diversity of populations existed the high level polymorphism.(2)The population genetic structure was assessed that all populations were classified into two different genetic clusters.(3)Analysis of molecular variance (AMOVA) and clustering analysis indicated showed that the most of the genetic variation within populations (93%),more than that among populations (7%).and there is a low level of population differentiation.The paper suggests,the genetic diversity of E.ulmoides existed the high level polymorphism.E.ulmoides has low genetic differentiation,and high similarity within populations:A high genetic differentiation within populations was revealed,suggesting that more individuals from different populations should be collected to protect the genetic diversity of E.ulmoides.

Eucommia ulmoides Oliv.SSRgenetic diversitygenetic structure

李洪果、贾宏炎、李武志、杜红岩、乌云塔娜、刘攀峰、包文泉

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中国林科院 热带林业实验中心,广西凭祥532600

国家林业局 泡桐研究开发中心,河南郑州450000

杜仲 SSR 遗传多样性 遗传结构

“十三五”国家重点研发计划项目

2017YFD0600702

2018

中南林业科技大学学报
中南林业科技大学

中南林业科技大学学报

CSTPCDCSCD北大核心
影响因子:1.442
ISSN:1673-923X
年,卷(期):2018.38(5)
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