Effects of intercropping Camellia oleifera and Carya illinoinensis on soil nutrients and fungal diversity
[Objective]The effect of the intercropping of Camellia oleifera and Carya illinoinensis on soil nutrients and fungal diversity was to be discussed.[Method]The main physical and chemical properties and enzyme activities,the structure and diversity of fungal community,and the correlation between soil environmental factors of 0-30 cm forest soil and fungi were analyzed by using technologies such as Illumina Miseq high-throughput sequencing in three forest modes,which were C.oleifera monocropping model(Cam),C.oleifera and C.illinoinensis intercropping model(Cam&Car),and C.illinoinensis monocropping model(Car).[Result]The soil pH value and the content of hydrolytic nitrogen,available phosphorus and organic matter in the intercropping forest were between the C.oleifera and C.illinoinensis monocropping model.The soil enzyme activity increased partly but the content of available potassium(AK),available sulfur(AS),exchangeable calcium(ECa)decreased in the C.illinoinensis monocropping model.A total of 1 084 OTUs were obtained by sequencing of soil fungi in three modes.The number of OTUs shared by the three modes was 111.And the order of unique OTUs in three modes was Car＞Cam&Car＞Cam.A total of 257 species of fungi belonging to 8 phyla,11 classes,56 orders,131 families,201 genera were identified from three modes,among which Saccharomycetes,Sordariomycetes and Agaricomycetes were the common dominant fungi class of the three modes,and Wickerhamyces was the common dominant fungi.Alpha diversity analysis showed that the ACE index of soil fungi in three modes had differences,which was Car＞Cam&Car＞Cam,while Shannon index and Simpson index had no significant difference.Principal coordinate analysis based on Bray Curtis distance showed that Cam&Car intercropping model intersected with Car monocropping mode and Cam monocropping model,and more intersections with Car monocropping model.The results of correlation analysis showed that there was a correlation between the main fungi at the genus level and the soil environment,among which there was a significant negative correlation with the content of exchangeable calcium,available potassium and available sulfur.[Conclusion]The intercropping of C.oleifera and C.illinoinensis increased the nutrient consumption of forest soil,and the soil enzyme activity and microbial diversity of C.illinoinensis forest partly.The intercropping of C.oleifera and C.illinoinensis should increase fertility management,especially the input of fertilizers containing calcium,potassium and sulfur.
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