Effects of alkali stress on physiology and biochemistry of different genotypes seedlings of×Taxodiomera peizhongii
[Objective]This research investigated the effects of alkali stress on physiology and biochemistry of different genotypes of×Taxodiomera peizhongii seedlings,to evaluate the alkali tolerance ability of different genotypes of seedlings,and provide reference basis for the study of alkali tolerance mechanism and the promotion and application of×T.peizhongii seedlings.[Method]The experiment used 2.5-age-old cuttage container seedlings of different genotypes of×T.peizhongii(S1,S2,S3,S4 and S5)as materials,which were treated with different concentrations alkaline salt solution(pH8,pH9 and pH10).The physiology indicators were measured to analyze the alkali tolerance ability among five genotypes of×T.peizhongii such as the content of relative conductivity,soluble sugar content,SOD activity,MDA content,chlorophyll content and chlorophyll a/b.The alkali tolerance of five genotypes was comprehensively evaluated by membership function method.[Result]The effects of different genotypes,different alkali treatments,and the interaction of genotypes and alkali on the relative electrical conductivity,soluble sugar content,SOD activity,MDA content,chlorophyll content,genotype and the interaction between genotype and alkali on the chlorophyll a/b ratio×T.peizhongii reached an extremely significant difference(P＜0.01).With the extension of treatment time,the relative conductivity,soluble sugar content,SOD activity,MDA content,chlorophyll content and chlorophyll a/b of S1,S3 and S5 genotypes of×T.peizhongii seedlings increased first and then decreased under alkali treatment.After 57 days of treatment,the relative conductivity,SOD activity and MDA content of the leaves of the five genotypes of×T.peizhongii under pH10 treatment were significantly higher than those under CK treatment,but the chlorophyll content and chlorophyll a/b value of the leaves of the five genotypes were not significantly different.Integrated analysis of fuzzy membership function values showed that the relationship of alkali resistance of five genotype was:S5＞S4＞S3＞S1＞S2.[Conclusion]Different genotypes seedlings of×T.peizhongii regulated their physiology to cope with alkali stress quickly under different alkali stress treatment.However,there were differences in the alkaline resistance of different genotypes of×T.peizhongii seedlings,which were reflected in the differences in dynamic changes of physiological and biochemical indicators.Meanwhile,the comprehensive evaluation of the physiological index test results showed that the S5 genotype seedlings had the strongest alkali resistance.
×Taxodiomera peizhongiigenotypealkaline stressphysiological and biochemical
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