Tissue culture and rapid propagation technology of Acer truncatum
[Objective]Acer truncatum is a unique woody oil plant in China,which is very suitable for development and utilization,but it is very difficult to propagate and root ex vivo in tissue culture,establish a reasonable complete tissue culture ex vivo regeneration system,obtain a large number of seedlings and the current year's bud stem segment in a short period of time,which will provide a new way for the large-scale production of excellent seedlings of this species,and provide a research basis for the selection and breeding of A.truncatum seeds,the preservation and promotion of germplasm resources,etc.[Method]The germ segments and seeds of A.truncatum were used as the test materials,and the sterilization method and axillary bud induction,sub-proliferation and rooting medium were designed and screened by orthogonal design screening method and univariate screening method.Different concentrations of 6-BA,NAA and TDZ hormones were added to MS,1/2MS and NN69 basal medium,respectively,and the most suitable medium for each stage of A.truncatum was found through different ratios.[Result]The best collection time for bud stem segments in April of the year,after brushing the surface and rinsing for half an hour with washing powder solution,the sterilization effect was best with 75%alcohol 30 s + 0.1%HgCl2 soaked for 10 min.The most suitable medium at each stage of the regeneration system was MS basal medium,and the addition of different concentrations of hormones on the basis could induce the differentiation of young shoots into different organs.At the beginning of the culture stage,6-BA had the greatest effect on axillary bud induction,and the induction rate of optimal medium could reach 73.33%.In the secondary proliferation stage,TDZ had the greatest effect on adventitious bud differentiation,with a proliferation coefficient of up to 4.31,and a univariate screening method was used in the rooting stage,and it was found that 0.2 mg·L-1 IBA had the highest rooting rate and rooting number,reaching 93.33%and 4.3.[Conclusion]The optimal medium for A.truncatum is 1)Initiation culture:MS + 0.06 mg·L-1 6-BA and 0.06 mg·L-1 NAA,induction rate was 73.33%;2)proliferation culture:MS+ 0.3 mg·L-1 TDZ and 0.05 mg·L-1 NAA;3)Rooting culture:MS+0.2 mg·L-1 IBA.
NETL
NSTL
万方数据
