摘要
[目的]以辽东栎不同大小种子幼苗为材料,研究了子叶去除对幼苗生长的影响及施氮的生长补偿效应,以期为辽东栎实生更新和苗木繁育提供参考.[方法]采用盆栽方法,设置子叶去除和施氮(40 g·m-2·a-1)处理,测定幼苗株高、主根长、单株叶面积、总干质量等指标,并计算根冠比、比叶面积、比根长、比枝长、相对生长率和净同化率等参数.通过三因素方差分析比较各处理间的差异.[结果]1)子叶去除显著减小辽东栎大小种子幼苗株高、主根长、单株叶面积、总干质量,比枝长、相对生长率和净同化率,增大了辽东栎大小种子幼苗根冠比、比叶面积和比根长,且在各处理中,大种子幼苗株高、单株叶面积、总干质量、比叶面积显著大于小种子幼苗;2)施氮对子叶去除辽东栎大小种子幼苗株高、主根长、单株叶面积、总干质量、比根长、相对生长率和净同化率有补偿作用,且子叶去除后施氮处理辽东栎大小种子幼苗单株叶面积、总干质量、比叶面积、相对生长率和净同化率显著大于子叶去除后不施氮处理辽东栎大小种子幼苗.[结论]子叶去除对辽东栎小种子幼苗的影响大于大种子幼苗,施氮在一定程度上对子叶去除辽东栎大小种子幼苗的生长有补偿作用,但不能代替子叶在幼苗生长发育过程中的重要作用.
Abstract
[Objective]In this study,the effects of cotyledon removal on the growth of Quercus wutaishanica seedlings and the growth compensation effect of nitrogen application were studied,in order to provide reference for the growth regeneration and seedling breeding of Q.wutaishanica.[Method]Cotyledon removal and nitrogen application(40 g·m-2·a-1)were used to determine the shoot height,root height,leaf area per plant and total dry mass,and the root-shoot ratio,specific leaf area,specific shoot length,specific root length,relative growth rate and net assimilation rate were calculated.The differences among the treatments were compared by three-factor analysis of variance.[Result]1)Cotyledon removal significantly reduced shoot height,root length,leaf area per plant,total mass,specific shoot length,relative growth rate and net assimilation rate,and increased the root-to-shoot ratio,specific leaf area and specific root length of large and small seed seedlings.In each treatment,shoot height,leaf area per plant,total mass and specific leaf area of large and small seed seedlings were significantly higher than those of small seed seedlings;2)Nitrogen fertilization had compensatory effects on the removal of shoot height,root height,leaf area per plant,total mass,specific root length,relative growth rate and net assimilation rate by cotyledon.The leaf area per plant,total mass,specific leaf area,relative growth rate and net assimilation rate of Q.wutaishanica seed seedlings treated with nitrogen cotyledon removal were significantly higher than those treated without nitrogen cotyledon removal.[Conclusion]The effect of cotyledon removal on small seed seedlings of Q.wutaishanica was greater than that of large seed seedlings.Nitrogen application could compensate the growth of large and small seed seedlings of Q.wutaishanica to some extent,but it could not replace the important role of cotyledon in the growth and development of seedlings.
基金项目
国家自然科学基金项目(31660195)
宁夏回族自治区重点研发计划项目(2018BEG02001)
NETL
NSTL
万方数据