Effects of different hormone treatment and cutting times on rooting of Sapium sebiferum softwood cutting after juvenile treatment
[Objective]To optimize the experiment of cutting and seedling cultivation of Sapium sebiferum,and provide practical reference for its the asexual reproduction;Observing the morphology,anatomical structure,and nutrient changes during the rooting process provides a theoretical basis for the rooting mechanism of S.sebiferum.[Method]Six-year-old S.sebiferum was used as the material to analyze the effects of exogenous hormones(NAA of 200,500,800 mg·L-1 and IBA of 800,1 000,1 200 mg·L-1)and cutting period(June,September)on the rooting of S.sebiferum softwood cuttings after juvenile treatment;Regularly took photos for morphological observation to determine the sampling period,by using paraffin sectioning technology to observe the anatomical structure of cuttings,and the anthrone colorimetry and Coomassie brilliant blue method were used to determine the content of soluble sugar,starch,and soluble protein.[Result]Juvenile treatment significantly improved the rooting effect of S.sebiferum cuttings.The rooting effect of the IBA treatment group was significantly better than that of NAA,with the highest rooting rate reaching 100%when the concentration of IBA was 1 200 mg·L-1.The rooting indicators of S.sebiferum cuttings in June were higher than those in September.Anatomical observations did not reveal any latent root primordia,so the root of S.sebiferum tender branch cuttings may originate from the induced root primordia,with two types of root formation:skin and callus.During the rooting process of S.sebiferum cuttings,the starch content in the treatment group showed a trend of"down-up",while the soluble sugar and protein content showed a trend of"up-down-up".The change trend in the control group was similar to that in the hormone treatment group,but the peak appeared about 7 days later than in the treatment group.[Conclusion]The occurrence of adventitious roots in S.sebiferum belongs to the mixed rooting type.Juvenile treatment can effectively improve the rooting effect of S.sebiferum cuttings.In June,IBA hormone treatment with 1 200 mg·L-1 has the best rooting effect,and growth regulators can improve the accumulation and metabolism of nutrients,promote rooting of cuttings.
万方数据
维普
