Effects of humus on physiological and biochemical characters of Polygonatum cyrtonema leaves under drought stress
[Objective]By studying the changes of physiological and biochemical indicators of Polygonatum cyrtonema under drought stress,and comprehensively evaluating the impact of humus on its resistance to drought stress,a theoretical basis for water-saving cultivation of P.cyrtonema was provided.[Method]Using humus and loess(control)as the culture medium,the pot experiment was conducted to determine the relative water content,chlorophyll content,relative conductivity,malondialdehyde content,soluble sugar content of P.cyrtonema leaves by setting four water supply gradients:normal water supply(soil water content 80%-85%),mild drought(soil water content 60%-65%),moderate drought(soil water content 40%-45%),and severe drought(soil water content 20%-25%).Changes of physiological and biochemical indexes such as relative water content,chlorophyll content,relative conductivity,malondialdehyde content,soluble sugar content,soluble protein content,superoxide dismutase activity and peroxidase activity in P.cyrtonema leaves were measured.[Result]Mild drought stress did not cause significant changes in physiological and biochemical drought resistance indicators of P.cyrtonema leaves.Moderate drought stress had a significant impact on the physiology and biochemistry of P.cyrtonema leaves,among which the relative water content,chlorophyll content,SOD activity and POD activity in humus soil matrix were respectively decreased by 0.34%,4.99%,18.37%,2.90%less than those in the control matrix,but the relative conductivity and MDA content were respectively increased by 10.30%and 9.12%less than those in the control matrix,and the soluble sugar content and soluble protein content were respectively increased by 3.60%and 1.79%more than those in control matrix.Severe drought stress had a significant impact on the physiology and biochemistry of P.cyrtonema leaves,among which the relative water content,chlorophyll content,SOD activity,POD activity in Humus soil matrix were respectively decreased by 2.16%,5.14%,19.92%and 8.56%less than those in the control matrix,but the relative conductivity and MDA content were respectively increased by 14.16%and 16.86%less than those in the control matrix,and the soluble sugar content and soluble protein content were respectively increased 4.06%and 2.35%more than those in the control matrix.The comprehensive evaluation of the subordinate function showed that the drought resistance subordinate function value of P.cyrtonema in the humus soil substrate culture was 151%higher than that of the control substrate.[Conclusion]As a cultivation substrate for promoting the growth of P.cyrtonema,humus can improve the stability of physiological and biochemical drought resistance indicators of P.cyrtonema under drought stress,and can resist drought stress to a certain extent by increasing the content of osmoregulation substances.
humusPolygonatum cyrtonemaleavesdrought stressphysiology and biochemistry characters
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