Embryo proliferation and maturation culture of Quercus variabilis Blume var.pyramidalis
[Objective]In order to explore the influence of various factors on the development and maturation of Quercus variabilis Blume var.pyramidalis embryo,and further improve the embryogenesis system of Q.variabilis Blume var.pyramidalis.[Method]The main factors affecting the proliferation and maturation of Q.variabilis Blume var.pyramidalis embryo were explored by using the embryonic callus induced by Q.variabilis Blume var.pyramidalis embryo as materials:single-factor experiments were used to screen the types of light conditions(strong light,weak light,no light)and hormones(naphthaleneacetic acid(NAA),cytokinin 6-benzylaminopurine(6-BA),auxin 2,4-dichlorophenoxyacetic acid(2,4-D))suitable for the proliferation of Q.variabilis Blume var.pyramidalis embryo.The concentration and ratio of hormones affecting embryonic proliferation were analyzed by two-factor tests.Univariate experimental analysis was carried out on sucrose concentration,activated carbon concentration and ABA concentration affecting embryo maturation.Finally,a two-factor test was carried out on the concentration and ratio of hormones affecting embryonic proliferation.[Result]1)Among three different light intensities,the induction rate of embryonic proliferation was the highest in the strong light environment;2)There were significant differences in the proliferation of embryos by different plant hormone ratios,with the highest proliferation rate of 83.33%with 0.10 mg/L2,4-D and 0.80 mg/L 6-BA;3)When the sucrose concentration was 5.00%,the maturity rate of the embryo was the highest which was 77.80%;When the ABA concentration was 0.50 mg/L,the maturity rate reached up to 50.00%;When the concentration of activated carbon was 1.00g/L,the growth was good,and the maturity rate reached 57.10%;4)When the concentration of NAA and 6-BA was 0.25 mg/L at the same time,the maturity rate of embryo reached up to 90.50%.[Conclusion]1)The body embryo of Q.variabilis Blume var.pyramidalis has a good proliferation effect in a strong light environment;2)The optimal proliferation medium is:MS+(6.0 g/L)agar+(3.00%)sucrose+2,4-D(0.10 mg/L)+6-BA(0.80 mg/L);3)The optimal maturation medium is:MS+(7.0 g/L)agar+(5.00%)sucrose+NAA(0.25 mg/L)+6-BA(0.50 mg/L)+ABA(0.50 mg/L)+activated carbon(1.50 g/L).
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