Analysis of pollen morphology and pollen viability of thirteen Lagerstroemia indica varieties
[Objective]The study of pollen morphological characteristics and pollen vigor differences among varieties provides a good theoretical reference for the subsequent cross-breeding work of Lagerstroemia indica varieties.[Method]The surface morphology of the pollen grains of the stamen cluster of 13 L.indica varieties was observed and studied by scanning electron microscope,and 10 pollen grains of each varieties were randomly selected and statistically analyzed,while the pollen viability of the 13 L.indica varieties was determined by using solid culture medium.[Result]The pollen grains of the 13 L.indica varieties with short and long stamens were oblong spherical in equatorial view,with three germination grooves,cleft to close to the poles,not connected between the germination grooves,and obtusely triangular in outline in the polar view,and all of them belonged to the N3P4C5 type of pollen.The outer wall ornamentation of pollen grains,polar axis length,equatorial axis length,germination furrow characteristics and pollen grain volume size varied significantly among L.indica,with pollen size ranging from 538.91 to 1 402.40 μm2,polar axis length from 30.51 to 46.31 μm,equatorial axis length from 17.35 to 31.49 μm,germination furrow length from 18.38 to 34.36 μm,and the width of the germination furrow ridge from 5.72 to 11.34 μm.The outer wall of pollen mainly included three kinds of decorations:pore-like,brain-wrinkle-like and wrinkle-wave-like,and the surface of pollen grains of 13 test varieties with long stamens was smoother and fuller than that of pollen grains with short stamens.The results of pollen vigor determination showed that L.indica'Zijingling'had the strongest pollen vigor,with a pollen germination rate of 93.94%,among which L.indica'Xiangyun'was a sterile variety,whose sterility type was male sterility,and the pollen did not germinate.[Conclusion]Morphological characteristics of pollen grains and surface ornamentation of pollen grains of different varieties of L.indica can be used as one of the reference indexes for the classification and identification of L.indica varieties.The 13 varieties of L.indica in this experiment,except for the sterile variety of L.indica'Xiangyun',the other varieties of the experiment showed high pollen viability,and all of them can be used as the materials for the subsequent work of cross-breeding of L.indica varieties.
Lagerstroemia indicapollen morphologygroup of stamenspollen vitality
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