Differential gene expression analysis of Lingwuchangzao jujube fruit at different stages of root tiller and grafting reproduction
[Objective]The differences in the internal mechanism of fruit quality between root tiller reproduction and grafting reproduction of'Lingwuchangzao'jujube were studied to provide basic research for improving its fruit quality.[Method]The fruits of'Lingwuchangzao'jujube root tiller reproduction and grafting reproduction at white maturity stage(NB),coloring stage(NZ),maturity stage(NC)and grafting reproduction at white maturity stage(JB),coloring stage(JZ)and maturity stage(JC)were used as experimental materials.High-throughput sequencing technology was used to sequence the fruit transcriptome.The differential genes related to the quality difference of'Lingwuchangzao'jujube fruit of root tiller and grafting reproduction were screened,and the gene expression differences of the fruit of root tiller and grafting reproduction at different stages were preliminarily explored.[Result]The 18'Lingwuchangzao'jujube samples were subjected to transcriptome sequencing to obtain 138.26 GB of quality control data,which were aligned to the reference genome winter jujube.The cleans reads of the 18 samples were aligned with the reference genome.The efficiency was above 93.77%.The principal component analysis results showed that the three biological repeat samples had a higher degree of aggregation,and there was a separation phenomenon between the groups,indicating that there were differences in gene expression.After the difference analysis,the results showed that in the analysis and comparison of the fruit development of'Lingwuchangzao'jujube in each period,65,4 509 and 1 534 differentially expressed genes were screened out in the white ripening period(JB-vs-NB),coloring period(JZ-vs-NZ)and ripening period(JC-vs-NC),respectively.The GO function was enriched into 50 subcategories of three categories:biological process,molecular function and cellular component.The KEGG pathway annotation significantly enriched the differentially expressed genes in secondary metabolite biosynthesis,metabolic pathway,amino acid biosynthesis and other pathways.The differentially expressed genes in mature stage were significantly enriched in sugar and acid related pathways.[Conclusion]Among five metabolic pathways related to fruit soluble sugar and total acid content,starch and sucrose metabolism,galactose metabolism,amino sugar and nucleotide sugar metabolism,glyoxylic acid and dicarboxylic acid metabolism,and butyric acid metabolism,31 differential genes such as ncbi_107424266,ncbi_107414286,ncbi_107425230,ncbi_107429837 were screened out,which were differentially expressed in the sugar and acid metabolic pathways at different stages of fruit development,regulating the soluble sugar and total acid content in fruits at different stages.
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