Effects of drip fertilization on soil enzyme activities and bacterial community composition in Eucalyptus artificial forest
[Objective]Drip fertigation is an important measure to improve the productivity of Eucalyptus plantation,to understand the effects of drip fertilization on soil enzyme activity and bacterial community structure,and to provide certain theoretical basis for the scientific application of drip fertilization technology in the production of Eucalyptus plantation.[Method]We analysed the soil bacterial community in Eucalyptus artificial forest by 16s rRNA sequencing,and used physiological and biochemical methods to detect the characteristics of soil enzyme activities in response to drip fertilization.[Result]Enzyme activities of cellulase,acid phosphatase,sucrase,urease and chitin were significantly increased by 41.5%,12.5%,23.4%,28.4%and 25.7%under drip fertilization in the soil of Eucalyptus artificial forest.A total of 26 phyla,63 classes,127 orders,208 families,298 genera and 555 species of soil bacteria in Eucalyptus artificial forest.The phyla of Acidobacteria,Proteobacteria,Chloroflexi and Actinobacteria were dominated,which were mainly involved in metabolic function.Compared non-fertilization treatment,the bacterial diversity increased significantly under drip fertilization in the Eucalyptus artificial forest,and the relative abundance of dominant bacteria differed significantly.The relative abundance of Proteobacteria,Actinobacteria and Firmicutes was significantly increased under drip fertilization and Acidobacteria was significantly reduced.At the order level,there was a significant enrichment of nitrogen and potassium cycling related bacteria such as Rhizobiales,Caulobacterales,Sphingomonadales,and Halanaerobiales increased significantly.Moreover,soil pH,total nitrogen content and water content were the main factors affecting cellulase,acid phosphatase,sucrase,urease,chitin and soil bacterial communities.[Conclusion]Drip fertilization significantly increased the activities of a number of soil enzymes in Eucalyptus artificial forest,increased diversity of soil bacterial communities and enrichment of soil nitrogen and potassium nutrient cycling related soil bacteria,promoting soil nutrient cycling for eucalyptus growth.
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