Diversity characteristics of phyllosphere fungal communities and their influencing factors in Dacrydium pectinatum and Vatica mangachapoi
[Objective]Dacrydium pectinatum and Vatica mangachapoi are rare and endangered species in the tropical rainforests of Hainan Island.Investigating the diversity and structural composition of phyllosphere fungal communities and their environmental drivers in D.pectinatum and V.mangachapoi,which can provide a theoretical basis for the future utilization of the beneficial functions provided by microbial communities to improve the growth adaptability of rare and endangered plants.[Method]The high-throughput sequencing method was used to analyze phyllosphere fungal samples from D.pectinatum and V.mangachapoi,which explored the α and β diversity and community composition of the phyllosphere fungal communities and to elucidate their main environmental drivers.[Result]1)A total of 10 489 OTUs were analyzed and detected in this study,including 10 phyla and 40 orders of fungi.The leaf surface shared OTU was 523(4.99%)and the leaf inner shared OTU was only 9(0.09%)in D.pectinatum and V.mangachapoi.2)The dominant class included:Dothideomycetes,Eurotionmycetes,and Tremellomycetes.Among them,the relative abundance of Dothideomycetes on the leaf inner of V.mangachapoi was significantly greater than that of other species and compartments(P＜0.01).3)All leaf surface fungal α-diversity was higher than leaf inner,but the difference between phyllosphere fungal diversity was not significant across species,with species variation explaining 13.57%of the variation in the composition of the phyllosphere fungal community(P＜0.001).4)Phyllosphere fungal α-diversity showed a significant positive correlation with leaf pH and Temperature(pH:R2=0.156,P＜0.001;Tem:R2=0.164,P＜0.001),and also significantly negatively correlated with rainfall(Rain:R2=0.345,P＜0.001).The results of CCA analysis showed that Mg and rainfall explained 71.29%and 69.33%of the fungal community composition on the leaf surface,and pH and Mg explained 62.48%and 61.51%of the fungal community composition within the leaf inner,respectively.The PERMANOVA analysis indicated that total nitrogen(TN:R2=0.13,P=0.001),total phosphorus(TP:R2=0.12,P=0.001),Mg(R2=0.14,P=0.001),and Rainfall(Rain:R2=0.13,P=0.002)were the main drivers of the differences in the composition of the phyllosphere fungi of D.pectinatum and V.mangachapoi.[Conclusion]Species variation significantly affected the interleaf fungal community,with TN,TP and Mg being the main influences driving the differences in the composition of the phyllosphere fungal community between D.pectinatum and V.mangachapoi.
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