Tissue culture through the stem segment with buds of'Deyou No.6'Camellia oleifera
[Objective]To explore the effects of basic medium,hormone type and ratio on the bud and stem segment of Camellia oleifera belt,establish an efficient and rapid propagation system for C.oleifera in order to provide technical support for the commercial cultivation of C.oleifera in the future.[Method]The stem segment with bud of a superior C.oleifera plant'Deyou No.6'was used as the test material to study the effects of basic medium,growth hormone and hormone ratio on primary bud induction culture,IBA,IAA,KT and other hormone ratio on proliferation culture,and quick dip IBA,salicylic acid treatment methods on rooting culture inside and outside the bottle.Determine appropriate disinfection methods and breeding and rooting media.[Result]The axillary bud germination induced by IAA and IBA was significantly higher than NAA,and IAA was significantly better than IBA in promoting bud elongation.Both WPM and 1/2MS basic medium had good growth effect on bud induction,but WPM medium promoted the growth rate of bud.The orthogonal experiment of bud induction in stem segment of oil tea belt was designed with three hormones,6-BA,IAA and GA3,and the main factors were IAA＞6-BA＞GA3,A1B1C1:WPM+1.0 mg/L 6-BA+1.0 mg/L IAA+2.0 mg/L GA3,and the induction rate was 93.8%.With WPM+2.0 mg/L 6-BA+0.25 mg/L IBA+1.0 mg/L KT,the proliferation coefficient was 3.14,and the leaves were strong and green.For single bud treatment,the rooting effect was obvious when the base of the bud was rapidly soaked with 5.0 mg/mL IBA(10 s)+1.0 mg/L salicylic acid(20 s).The rooting rate reached 90.9%when the blank medium of 1/2MS(4g/L Agar)was added,and the rooting rate reached 87.5%when the medium of vermiculite+perlite was 1∶1.[Conclusion]The tissue culture and rapid propagation system of buds and stems of C.oleifera was established,especially the method of rapid dipping with high concentration of auxin was used to achieve a higher rooting rate by rooting inside and outside the bottle.
Camellia oleiferatissue culturestem segment with budinductionclustered seedlingsrooting
NETL
NSTL
万方数据
