Effects of formula fertilization on the growth and physiological characteristics of seedlings from different provenances of Phellodendron chinense Schneid
[Objective]To explore the effects of different formula fertilization on the growth and physiological characteristics of seedlings from three Phellodendron chinense Schneid provenances,aimed at providing suitable provenance and fertilization schemes for the scientific application of P.chinense Schneid.[Method]One-year-old seedlings from three P.chinense Schneid provenances were used as experimental materials.A L27(313)orthogonal experimental design was adopted to conduct ratio fertilization experiments on the three provenances.Growth indicators(seedling height,ground diameter)and physiological characteristics(chlorophyll,soluble sugar,soluble protein,malondialdehyde(MDA),superoxide dismutase(SOD),nitrate reductase(NR),and glutamine synthetase(GS))were measured.Correlation analysis and the subordinate function fuzzy evaluation method were used for comprehensive evaluation.[Result]Compared with the control(CK),formula fertilization significantly promoted the growth of seedling height and ground diameter,and the chlorophyll content of leaves.It enhanced the levels of soluble sugar,soluble protein,and enzyme activities(SOD,NR,GS),and significantly reduced the content of malondialdehyde,indicating a clear effect in inhibiting lipid peroxidation.The comprehensive evaluation of the subordinate function showed that the worst overall performance among the three provenances was CK,with the Chongqing Jiangjin provenance exhibiting the best comprehensive performance under T6(N2P3K1)treatment,the Hubei Enshi provenance performing best under T4(N2P1K2)treatment,and the Hunan Xiangxi provenance excelling under T8(N3P2K1)treatment.[Conclusion]Reasonable nitrogen,phosphorus,and potassium formula fertilization can improve the physiological metabolism level of P.chinense Schneid seedlings and promote their growth and development.The study indicates that among three provenances,the Hubei Enshi provenance exhibits the best overall performance,making it the preferred choice for cultivation,followed by the Hunan Xiangxi and Chongqing Jiangjin provenances.Under the conditions of this experiment,the optimal nitrogen,phosphorus,and potassium application rates for Chongqing Jiangjin provenance in production are 6 g·plant-1 of urea,5 g·plant-1 of superphosphate,and 2 g·plant-1 of potassium chloride.For the Hubei Enshi provenance,the optimal rates are 6 g·plant-1 of urea,1 g·plant-1 of superphosphate,and 3 g·plant-1 of potassium chloride.For the Hunan Xiangxi provenance,the optimal rates are 8 g·plant-1 of urea,3 g·plant-1 of superphosphate,and 2 g·plant-1 of potassium chloride.