Effects of shading on growth and biochemical characteristics of Gleditsia sinensis seedlings
[Objective]The objective of this study is to investigate the response mechanism of different shades to the growth and physiological response of Gleditsia sinensis seedlings,in order to provide a theoretical basis for the cultivation of high-quality G.sinensis seedlings.[Method]Half-year-old potted G.sinensis seedlings were used as materials,and four treatments were set up:full light(S0),30%shading rate(S1),60%shading rate(S2)and 90%shading rate(S3),and the changes of seedling height,ground diameter,new length,chlorophyll content,chlorophyll fluorescence parameters,soluble sugar SS,proline Pro,malondialdehyde MDA,superoxide dismutase SOD,catalase CAT and peroxidase POD were measured and analyzed.[Result]1)There were significant differences in the growth of seedling height,ground diameter and new length of G.sinensis seedlings under different shading environments,and the height and diameter ratio of G.sinensis seedlings was the smallest under S0 treatment,and the seedlings were relatively robust,and the seedlings were the largest under S3 treatment,and the seedlings were relatively slender;2)The chlorophyll content increased with the increase of shading degree,chlorophyll a/b Chla/b had no obvious change trend,the maximum photochemical efficiency Fv/Fm value was greater than 0.75,and the G.sinensis seedlings were not photo-inhibited under shading treatment;3)The SS content increased with the increase of shading degree,and the content of Pro in the S0 group decreased rapidly with the increase of shading stress time,and the Pro content of S0 in June was 1.45 times and 3.83 times that in July and August,respectively,and the shading treatment group showed a trend of first decreasing and then increasing.S1,S2 and S3 in August increased by 17.81%,58.35%and 77.15%respectively compared with those in June;4)The content of MDA was generally lower than S0 in shading treatment,and the changes of POD activity were opposite to that of MDA,and the activities of SOD and CAT decreased first and then increased with the extension of stress time;5)The mean membership degrees of S0,S1,S2 and S3 were 0.083 6,0.410 3,0.706 4 and 0.725 9,respectively,and the average membership of G.sinensis seedlings under S2(60%shade)and S3(90%shade)treatments were higher,indicating that the comprehensive growth of seedlings under this treatment was the best.[Conclusion]The comprehensive evaluation showed that the seedling quality of G.sinensis seedlings was the best under S2 and S3 shading conditions,and the cultivation and shading of G.sinensis seedlings was 60%-90%.
Gleditsia sinensisshade intensitygrowth traitsphotosynthetic propertiesphysiology and biochemistry
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