Salt tolerance and drought resistance function of ThZFP3 gene in Tamarix hispida
[Objective]Zinc finger proteins are a major class of important transcription regulatory factors in plants,playing important roles in plant growth and development,and stress response.The salt tolerance and drought resistance function of ThZFP3 of Tamarix hispida zinc finger protein gene was analyzed to provide a theoretical basis for genetic improvement of tree resistance.[Method]Arabidopsis thaliana was transformed with ThZFP3 using the floral dip method.The germination survival rate,root length,fresh mass,and phenotypic changes of the transgenic and control A.thaliana were observed under NaCl and mannitol stress.Transient overexpression(OE),inhibited expression(RNAi)and control transgenic Tamarix were obtained using the Agrobacterium transient transformation system.Three transgenic Tamarix plants were histochemically stained and stress-related physiological indexes were measured to identify the drought resistance and salt tolerance of the ThZFP3 gene.[Result]Under stress conditions,the germination survival rate,root length,fresh mass,and growth vigor of transgenic Arabidopsis were superior to wild-type strains,indicating that the ThZFP3 gene improved the drought and salt tolerance of transgenic Arabidopsis.Overexpression of the ThZFP3 gene significantly enhanced the activities of POD and SOD in T.hispida,thereby reducing the accumulation of reactive oxygen species(ROS);Overexpression of the ThZFP3 gene reduced the accumulation of MDA and electrolyte permeability in T.hispida,protecting the integrity of cell membranes.[Conclusion]Overexpression of the ThZFP3 gene can improve the ROS scavenging ability within the body of T.hispida,reduce the degree of cell damage,and thus improve its drought resistance and salt tolerance.
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