Physiological response and cold resistance evaluation of Sapindus mukorossi seedlings from four provenances to low temperature stress
[Objective]By studying the changes of physiological and biochemical indexes of Sapindus mukorossi from different provenances under low temperature stress,the cold resistance of S.mukorossi from different provenances was comprehensively evaluated,and cold-resistant provenances were selected,so as to provide theoretical basis for the introduction of S.mukorossi to the north.[Method]The cold storage simulated low temperature stress method was used to conduct pot experiment on S.mukorossi seedlings from 4 provenances,and 8 time gradient was set at 0(CK),2,4,8,12,24,48,72 h,and 11 physiological and biochemical indexes were measured,such as relative conductivity,relative water content,antioxidant enzyme system and osmotic regulatory substances.The membership value of each index was used to evaluate the ability of S.mukorossi from different provenances to resist low temperature stress.[Result]Physiological indexes of S.mukorossi from different provenances had different degrees of response to low temperature stress.The relative conductivity and MDA content increased with the increase of low temperature stress treatment time,while the relative water content decreased first and then increased.Compared with the relative water content and MDA content,the relative conductivity was more sensitive to low temperature stress.With the extension of low temperature stress time,the activities of SOD,POD,GR and free proline content of S.mukorossi leaf increased first and then decreased,the activity of CAT decreased first and then increased and then decreased,and the activity of APX,soluble sugar and soluble protein contents increased.Therefore,the S.mukorossi of 4 provenances can enhance cold resistance by activating their own protective enzyme system and osmotic regulatory substances.[Conclusion]The average membership value of cold-resistance related indexes of S.mukorossi from different provenances under low temperature stress was calculated according to membership function method.The low temperature tolerance of S.mukorossi was ranked from strong to weak as Hefei,Zhejiang,Jiujiang,Jiangxi and Xinxiang,Henan.
Sapindus mukorossileaveslow temperature stressphysiological responsegeographical provenanceevaluation of cold resistance
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