Analysis of gene expression related to radial growth of Eucalyptus cloeziana
[Objective]The study was intended to study the gene expression profile of different diameter grade of Eucalyptus cloeziana to reveal the mechanism of radial growth at the gene expression level.[Method]We sequenced the cambium transcriptomes of five different diameter grades E.cloeziana using the Illumina HiSeq 2000 sequencing platform.Gene expression level was calculated using featureCount software.Difference expression and expression pattern analysis were performed by edgeR and TCseq.[Result]A total of 1 364 837 824 high-quality reads were obtained after quality control,with an average GC content of 51.07%and an average alignment rate of 68.49%to the reference genome.Difference expression analysis of all comparison groups screened 8 794 different expressed genes(DEGs).3 292,3 876,3 604 and 4 565 DEGs were screened in the comparison ultra-high diameter group,large diameter group,small diameter group,ultra-low diameter group Vs medium diameter group,respectively,and with 1 878 up-regulated and 1 414 down-regulated,2 314 up-regulated and 1 562 down-regulated,1 599 up-regulated and 2 005 down-regulated,2 850 up-regulated and 1 715 down-regulated in these comparison,respectively.GO and KEGG analysis showed that DEGs were mainly enriched in glycosyltransferase activity,organocyclic compound binding,oxidorereductase activity,and were reported to be involved in cytochrome P450 metabolism,phenylpropane biosynthesis,phenylalanine metabolism,flavonoid biosynthesis and other metabolic pathways related to secondary xylem formation.Gene expression pattern analysis clustered all genes into 10 patterns,among which the genes in pattern 9 showed a downward trend with the decrease of diameter and were mainly enriched in Hippo signaling pathway and terpenoid backbone biosynthesis.[Conclusion]The radial growth of E.cloeziana is related to multiple metabolic pathways,among which the phenylalanine metabolic pathway related to lignin synthesis may be an important one,and the Hippo signaling pathway may be involved in the regulation of diameter size.
万方数据
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