Diversity analysis of leaf phenotypic traits in 50 camellias species
[Objective]This study is to provide a theoretical basis for genetic improvement and taxonomic identification of camellia germplasm resources.[Method]The leaf phenotypic trait index of 50 camellias species were determined,and the analysis was carried out by using image feature analysis,correlation analysis,principal component analysis and cluster analysis.[Result]1)Based on the values of leaf area and leaf circumference,the leaves of 50 camellias were preliminarily divided into three types:large,medium,and small,among them,medium leaves(52%)accounted for the majority,followed by small leaves(32%)and large leaves(16%);2)According to the aspect ratio,roundness,and circumference ratio,the leaf shape was divided into four categories:elliptical(42%)＞oval(38%)＞oblong(12%)＞lanceolate(8%),so the leaf shape is mainly elliptical followed by oval,and a few were oblong and lanceolate;3)Correlation analysis showed that there was a significant negative correlation between the circumference ratio and leaf area,leaf circumference,roundness,and aspect ratio(P＜0.01);There was a significant positive correlation between leaf area,leaf circumference,roundness,and aspect ratio(P＜0.01);4)Extracting two principal components,with a cumulative contribution rate of 97.688%,it was supposed that leaf phenotypic traits can be a classification criteria for camellia;5)The variation range of leaf traits of 50 camellias were 18.391%-52.157%,ranked as leaf area＞leaf circumference＞width to length ratio＞circumference to diameter ratio＞roundness;The diversity index(H)ranges from 1.702-1.925,with the highest H value(1.925)for the leaf area;6)Finally,based on cluster analysis,the 50 Camellias could be divided into four groups:group I was medium-sized oval leaves;Group II consisted of large oval or large elliptical leaves;Group III was small elliptical leaves,and group IV was small lanceolate leaves.[Conclusion]After long-term natural hybridization and artificial cultivation,Camellia not only appeared variations in flower phenotypes,but also have certain variations in leaf phenotypes,and the variations have corresponded regularly,which can provide a solid theoretical basis for the classification and identification of camellia plants in the later stage.
万方数据
维普
