Prediction of the anti-inflammatory Q-marker of Pharbitidis Semen based on fingerprint and network pharmacology
The fingerprint of Pharbitidis Semen was established by UPLC,and the main anti-inflammatory components were determined.Then,the network of"component-target-pathway"was constructed through the network pharmacology method,and molecular docking verifies the biological activity of Q-marker,and through the RAW264.7 cell experiment to verify the anti-inflammatory activity.16 batches of Pharbitidis Semen fingerprints were established,and 7 chromatographic peaks were identified,which were neochlorogenic acid,chlorogenic acid,caffeic acid,isoquercitrin,and isochlorogenic acid A,B&C.According to network pharmacology analysis,32 core targets and 6 key pathways of 7 chemical components were screened.Molecular docking ligands have strong binding ability to receptors.The results of NO inhibition rate clarified that the extract of Pharbitidis Semen and the 7 components have anti-inflammatory activity.Conclusion:Chlorogenic acid,neochlorogenic acid,caffeic acid,isoquercitrin,and isochlorogenic acid A,B&C are potential anti-inflammatory Q-markers of Pharbitidis Semen,which can provide the theoretical basis for its quality control and rational application.
国家科技期刊平台
NSTL
万方数据
维普
