Preparation and properties of carbon dots based on fructose and dopamine
Nitrogen doped carbon dots(N-CDs)were synthesized via one-step hydrothermal method using fructose as carbon source and dopamine(DA)as nitrogen source.The structure,morphology,surface functional groups and optical properties of N-CDs were characterized via X-ray diffraction(XRD),high-resolution transmission electron microscopy(HRTEM),X-ray photoelectron spectrometer(XPS),ultraviolet-visible absorption spectrum(UV-vis),Fourier-transform infrared spectroscopy(FT-IR)and other characterization techniques.The cytotoxicity was studied by MTT assay,and cell imaging performance was studied by confocal microscopy.The results showed that the N-CDs were spherical particles with an average particle size of about 1.76 nm.The surface was rich in functional groups,such as hydroxyl,amino,carboxyl and carbonyl groups.The fluorescence excitation and emission wavelengths of the N-CDs were 440 nm and 536 nm,respectively.The N-CDs demonstrated good water solubility and low cytotoxicity.At a concentration of 0.5 mg∙mL-1,the survival rate of Hela cells was higher than 93%.N-CDs could easily enter the cytoplasm through the cell membrane and showed bright fluorescence signal in the cell.This method is simple,green and environmentally friendly.The N-CDs have low cytotoxicity and can be used in intracellular fluorescence imaging,which is highly desirable for the early diagnosis of cancer cells.
国家科技期刊平台
NSTL
万方数据
维普
