In this experiment,the orthogonal test was used to extract the flavonoid from the leaves of Cleisto-calyx operculatus and the antioxidant activity of the flavonoids from the leaves was determined.The results showed that the optimal extraction conditions were as follows:ethanol concentration of 40%,solid-liquid ratio 1:45(g/mL),extraction time 40 minutes,and the extraction rate of flavonoids was 5.24%.The antioxidant activity test showed that in a certain range of concentration,the antioxidant activity increased with the increase of the con-centration of flavone extract,and the total reductive activity and DPPH scavenging activity were similar to Vc.Scavenging of nitrite and hydroxyl radicals is slightly weaker than Vc.
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