A Study of the Preparation and Stability of Chitosan Modified Liposome
In order to improve the stability of liposome,the coated liposome was prepared by ethanol injec-tion.With particle size as the index,through the optimization of single factor and orthogonal experiments,the key process was determined as:the soybean lecithin concentration is 10 mg/mL,chitosan concentration is 150 mg/mL,shear speed is 7000 r/min,shear time is 2 min,and ultrasonic time is 10 min,and the particle size of lipo-somes prepared by this process was 711.38 nm.The effects of four stabilizers(1%,soybean lecithin mass ratio),such as sodium caseinate,octenyl succinic anhydride,Tween 20 and Tween 80,etc.,on the particle size of lipo-some wall materials were compared,the result of which showed that Tween 80 offered the best effect.In this study,a kind of coated liposome with high stability and small particle size was obtained.
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