Effects of Rhodiola on Intraocular Pressure,Retinal Structure and PI3K/AKT/HIF-1α Signaling Pathway in Tail-suspended Mice
In view of the problems of increased intraocular pressure and retinopathy caused by space-related eye syndrome in microgravity environment,the effects and mechanisms of Rhodiola on intraocular pressure and retinal structure in tail-suspended mice were studied.In the experiment,55 C57 mice were divided into 5 groups,with 11 mice in each group,and were modeled for 2 weeks and administered for 4 weeks,which lasted for 6 weeks.The intraocular pressure of mice was meas-ured at the 2nd,4th and 6th weeks,and the retinal structure of mice was observed by HE staining after 6 weeks,and the expression levels of PI3K,AKT and HIF-1α genes in the retina of mice were measured by RT-PCR.The results showed that the intraocular pressure of mice was significantly higher at 4 weeks(P＜0.01)and decreased naturally at 6 weeks,and there were no obvious patho-logical changes in the retinal structure of mice at 6 weeks,and the expression levels of PI3K,AKT and HIF-1α genes in the retina were significantly increased(P＜0.05),and the intraocular pressure of mice with Rhodiola intervention could be effectively reduced at 4 weeks(P＜0.01),and PI3K,AKT,and the expression level of HIF-1α gene(P＜0.05)was significantly higher at 6 weeks com-pared with that in the blank group(P＜0.05).It is proved that Rhodiola can reduce the increase of intraocular pressure and regulate the expression level of PI3K/AKT/HIF-1α signaling pathway in mice,and the safety of low-dose Rhodiola intervention is better.
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