首页|MicroRNA (miR396) negatively regulates expression of ceramidase-like genes in Arabidopsis
MicroRNA (miR396) negatively regulates expression of ceramidase-like genes in Arabidopsis
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
维普
MicroRNAs (miRNAs) are 21-23 nucleotide (nt), endogenous RNAs that regulate gene expression by targeting mRNAs for direct cleavage or translational repression in plants. In Arabidopsis, miR396 is encoded by two different loci (MIR396a and M1R396b) and sequence analysis suggests it may target three ceramidase-like genes (Atceramidase-like 1, Atceramidase-like 2 and Atceramidase-like 3). To demonstrate the biological function of miR396, we inserted the synthetic precursors, MIR396a or MIR396b, under the control of the enhanced cauliflower mosaic virus (CaMV) 35S promoter, into a plant transformation vector (pOCA30) and transformed the con-structs into Arabidopsis. The promoter increased miR396 levels by more than 2-fold, indicating appropriate maturation of the synthetic precursor MIR396a or MIR396b transcript in transgenic plants. Microarray analysis showed that the transcript levels of two ceramidase-like genes (Atceramidase-like 1, Atceramidase-like 2) were decreased by more than 2-fold and lactosylceramide 4-α-galactosyltransferase increased by more than 2-fold in transgenic plants compared with the empty vector-transformed plants. Northern blot analysis showed that the mRNA levels of the two ceramidase-like genes were significantly reduced in transgenic plants. These results indicated that miR396 probably plays a crucial role in the ceramide metabolism pathway by negatively regulating the expression of ceramidase-like genes in Arabidopsis.
miR396miR396 precursorCeramidase, Ceramide
Dongmei Liu、Diqiu Yu
展开 >
Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China
Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
国家高技术研究发展计划(863计划)国家自然科学基金Science Foundation of Yunnan Province
NETL
NSTL
万方数据
维普
