Identification of the role of the pyroptosis related gene for bladder cancer:A study based on TCGA database
Objective:To identify key pyroptosis-related genes(PRGs)in patients with bladder cancer(BLCA)by performing bioinformatics analysis and to clarify their potential value.Methods:We downloaded the mRNA expression profiles and clinical data corresponding to bladder samples from The Cancer Genome Atlas(TCGA)database.Next,we performed functional enrichment analyses,including both Gene Ontology(GO)and Kyoto En-cyclopedia of Genes and Genomes(KEGG)and analyses,to evaluate the mechanism underlying the signature.In addition,a comprehensive bioinformatics analysis was performed,and we constructed a prognostic gene model.The correlations between PRGs and tumor immune infiltration and microsatellite instability were evaluated.Moreover,we constructed a competing endogenous RNA(ceRNA)regulatory axis based on their coexpression and predicted targets.Results:We selected 33 PRGs,and functional enrichment analyses revealed these PRGs are mainly involved in the positive regulation of cytokine production,inflammasome complexes,apoptotic pro-cesses,and NOD-like receptor signaling pathways.A prognostic PRG model constructed using the 6 prognostic genes predicted the overall survival(OS)of BLCA patients with medium-to-high accuracy.The genetic muta-tion landscape of the PRGs in BLCA was also summarized.A significant correlation was observed between prognostic PRGs(AIM2 and CASP6)and immune cell infiltration and microsatellite instability.A ceRNA net-work was constructed and identified the CASP6/hsa-let-7c-5p/MIR29B2CHG regulatory axis in BLCA.Conclu-sion:The prognostic PRGs were significantly correlated with immune cell infiltration and microsatellite insta-bility in BLCA patients.A CASP6/hsa-let-7c-5p/MIR29B2CHG regulatory axis for the BLCA are needed to verify.
pyroptosis related geneprognosticimmune infiltrationregulatory axisbladder cancer
万方数据
维普
