Research on the correlation between gut microbiota and Traditional Chinese Medicine syndrome types in patients with colon polyps
Objective:The fecal and colonic mucosal microbiota of patients with colorectal polyps of different TCM syndromes were analyzed to explore the correlation between gut microbiota and TCM syndrome types.Methods:A total of 194 patients with colorectal polyps were included.According to the symptoms,tongue and pulse,all patients were divided into four TCM syndrome types:55 cases of large intestine damp-heat syn-drome in group A,54 cases of spleen and stomach weakness syndrome in group B,58 cases of liver-qi stag-nation syndrome in group C,and 27 cases of cold-dampness-blocking syndrome in group D.All patients com-pleted questionnaires on basic information,lifestyle and diet habits,and fresh feces were collected.Samples of normal sigmoid mucosa were also collected during electropolypectomy under colonoscopy.Fecal and mucosal microbiota were detected and analyzed by 16s rRNA sequencing.Results:There were no statistical differences in gender,age,BMI,diet regularity,exercise,size,number and pathological type of colon polyps among the four groups,while there were differences in smoking and dietary structure among the four groups(P=0.038;P=0.002).Fecal microbiota analysis showed that the Alpha diversity of group A and group B had a statistical difference(Chao:P=0.034,Richness:P=0.049).Mucosal microflora analysis showed that the Alpha diversity of group A and B was significantly different from that of group C(Chao:group A、C,P=0.004,groupB、C,P=0.012;Richness:group A、C,P=0.004,group B、C,P=0.011).Beta diversity of patients in the four groups showed similar clustering degree both of fecal microbiota and mucosal microbiota.However,there were differen-tial bacteria at genus level among different TCM syndrome groups.Both Beta diversity analysis and Anosim analysis indicated that fecal microbiota and mucosal microbiota were significantly different in all groups.Con-clusion:There are differences in gut microbiota of colorectal polyp patients with different TCM syndrome types,the characteristics of mucosal microbiota may be the microscopic manifestation of TCM syndromes.
colorectal polypstraditional Chinese medicine syndrome typesgut microbiota
NETL
NSTL
万方数据
维普
