Sialylated IgG is highly expressed in lung adenocarcinoma(LUAD)and promotes proliferation and mi-gration of LUAD in mice
0bjective:To investigate the expression and function of sialylated IgG(SIA-IgG)in LUAD,and the effect of anti-SIA-IgG antibody(RP215 antibody)on the growth and tumor microenvironment of Lewis LUAD in mice.Methods:Pan-cancer analysis of immunoglobulin heavy constant gamma(IGHG)genes was performed using The Cancer Genome Atlas(TCGA)database.The relationship between gene expression of IGHG and sur-vival prognosis of LUAD patients was analyzed using the Kaplan-Meier Plotter database.The expression of SIA-IgG in LUAD PC9 cells was silenced using shRNA,and the knockdown efficiency of SIA-IgG was de-tected by Western blot.Subsequently,clone formation assays and migration assays were utilized to evaluate the proliferation and migration capabilities of LUAD cells.Then,LLC cells were used to form tumors in female C57BL/6 mice(six weeks)and treated with RP215 antibody.The growth of tumors in the mice was observed,and the effect of RP215 treatment on tumor microenvironment was observed by flow cytometry.Results:IgG expression is upregulated in LUAD(P＜0.01)and correlates with poor prognosis.Silencing SIA-IgG expression significantly inhibits the proliferation(P＜0.01)and migration(P＜0.01)capabilities of LUAD cells.Subsequently,treatment with RP215 antibody markedly suppresses Lewis LUAD growth in mice(P＜0.05),and the propor-tion of macrophages and neutrophils in the tumor microenvironment of mice was reduced after RP215 treat-ment(P＜0.05).Conclusion:SIA-IgG may serve as a potential therapeutic target for LUAD.
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