The study of relationship between protease/antiprotease levels and different TCM syndrome types in stable chronic obstructive pulmonary disease of mice
Objective:To investigate the relationship between protease/antiprotease levels and different TCM syndrome types in stable chronic obstructive pulmonary disease of mice.Methods:A total of 60 healthy male BALB/c mice with SPF grade were randomly divided into 5 groups with 12 mice each.In addition to the blank control group,the combined mouse models of COPD with different syndrome types were established with syndrome modeling factors plus disease modeling factors,which were as follows:Blank control group,mod-el control group,COPD spleen deficiency syndrome group,COPD kidney deficiency syndrome group,COPD spleen and kidney deficiency syndrome group.Serum of mice was collected in batches at week 5,week 7 and week 15 to detect α-1 antitrypsin(AAT)/neutrophil elastase(NE)levels,and to analyze the dynamic changes of protease/antiprotease levels in mice with different syndromes,and to analyze the correlation between the chang-es of protease/antiprotease levels in mice with different syndromes by multiple disordered logistic regression.Results:The levels of AAT in the model control group,COPD spleen deficiency group and COPD kidney defi-ciency group were increased in different degrees compared with the blank group at the 15th week,but there was no significant statistical difference(P＞0.05).At the 15th week,NE was higher than that of the other four groups in blank group,and the levels of NE in spleen deficiency group and kidney deficiency group were higher than that in blank group(P＜0.05).Multivariate logistic regression correlation analysis showed that the level of NE was affected by spleen deficiency and kidney deficiency in mice at week 15.Conclusion:Com-bined with the results of inter-group comparison,it was suggested that spleen deficiency and kidney deficien-cy might mean higher anti-protease level.
stable stage of chronic obstructive pulmonary diseaseanimal experimentsprotease/antiprotease
万方数据
维普
