The effects of hyaluronic acids with different weight on osteogenic differentiation of periodontal liga-ment cells
Objective:To compare the biological reactions of human periodontal ligament cells(PDLCs)to four distinct molecular weights of hyaluronic acid(HA)directly.Methods:PDLCs were cultured in medium alone for 21 days(control group)or were added with osteogenic stimulation(OS group)medium.The other 4 experi-mental groups were added with different MW HAs in OS medium:ultra-low molecular weight hyaluronic acid group(ULMW HA),low molecular weight hyaluronic acid group(LMW HA),medium molecular weight hyal-uronic acid group(MMW HA)and high molecular weight hyaluronic acid group(HMW HA).The cell morpholo-gy was checked daily.The osteoblast differentiation and mineralization of PDLCs stimulated by different MW HAs were analyzed qualitatively and quantitatively by Von Kossa staining and calcium deposition.Results:Over time,cells stimulated with OS alone or with the addition of HAs showed the microscopic appearance of all typical osteogenic differentiation.After 21 days of stimulation,mineral and calcium deposits appeared in all groups except control group,and the LMW HA group showed the most significant calcium deposition.Conclu-sion:HA can promote the mineralization of PDLCs,in which LMW HA being the more effective.
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